Identifikasi Spesies Ikan Pelagis yang Dijual di Pasar Kota Denpasar Menggunakan Marka Control Region Mitokondria (mtDNA)
Keywords:
pelagic fish, diversity, BLAST, phylogeneticAbstract
Identification and inventarisation of fish species is important for fisheries data. Information regarding fish species sold within the local markets of Denpasar city-Bali have not been collected and written as a good data, especially identification using molecular methods and comparison between local name and species name. Therefore, this study aims to identify species of pelagic fish sold within markets of Denpasar city using control region mitochondrial (mtDNA) marker. A total of 7 samples of pelagic fish were collected from 2 markets within Denpasar city, in the form of full body fish, fish that has been boiled, and boiled fish body parts. DNA samples were amplified at the control region of mtDNA, and obtained the sequence length of 508-527 bp. Identification was conducted using BLAST methods and phylogenetic analysis. The result showed that the samples were identified as 4 species, i.e. Auxis rochei, Auxis thazard, Thunnus albacares, and Thunnus obesus. Through this study, we also revealed that fish with the local name “ikan tongkol” was the species of Auxis rochei, Auxis thazard, Thunnus albacares, meanwhile fish known as the local name “ikan cakalang” was the species of Thunnus obesus. The results of this study was expected to be a preliminary study about the diversity of fish sold within markets in Denpasar city, and also as the additional genetic data for pelagic fish within Bali waters. This will also act as the additional information for the methods of fish species identification.
References
Abdullah, A., & Rehbein, H. (2016). DNA Barcoding for The Species Identification of Commercially Important Fishery Products in Indonesian Markets. International Journal of Food Science and Technology, 52(1), 266-274. https://doi.org/10.1111/ijfs.13278.
Allen, G.R., Erdmann, M.W., & Pertiwi, N.P.D. (2022). Description of Three New Species of Damselfish Belonging to the Pomacentrus philippinus Group (Pomacentridae) from Melanesia and the Eastern and Central Indian Ocean. Aqua, International Journal of Ichtiology, 28(1), 1-26.
Asche, F., Garlock, T.M., Anderson, J.L., Bush, S.R., Smith, M.D., Anderson, C.M., Chu, J., Garrett, K.A., Lem, A., Lorenzen, K., Oglend, A., Tveteras, S., & Vannuccini, S. (2018). Three Pillars of Sustainability in Fisheries. PNAS, 115(44), 11221-11225. https://doi.org/10.1073/pnas.1807677115.
Astarini, I.A., Ningsih, E.Y., Simanungkalit, D., Ardiana, S.A., Malik, M.D.A., Yusmalinda, N.L.A., Sembiring, A., Pertiwi, N.P.D., Cahyani, N.K.D., & Collins, A. (2021). Genetic Variation of Longtail Tuna Thunnus tonggol Landed in Four Fish Markets in Indonesia based on Mitochondrial DNA. Biodiversitas, 22(4), 1644-1651. DOI: 10.13057/biodiv/d220408.
Babe, B.Y., Erfin, L., & Yohanista, M. (2021). Identifikasi Jenis – jenis Ikan Pelagis Kecil yang Ada di Pasar Alok dan Pasar Wuring, Kabupaten Sikka. Aquanipa-Jurnal Ilmu Kelautan dan Perikanan, 3(2), 1-18.
Cardenosa, D. (2019). Genetic Identification of Threatened Shark Species in Pet Food and Beuty Care Products. Conservation Genetics, 20, 1383-1387. https://doi.org/10.1007/s10592-019-01221-0.
Collette, B., Graves, J., & Kells, V.A. (2019). Tunas and Billfishes of the World. Johns Hopkins University Press. USA.
DeSalle, R., & Goldstein, P. (2019). Review and Interpretation of Trends in DNA Barcoding. Frontiers in Ecology and Evolution. 7, 302. doi: 10.3389/fevo.2019.00302.
FAO. (2020). The State of World Fisheries and Aquaculture 2020. Sustainability in Action. Rome. https://doi.org/10.4060/ca9229en.
Fitrah, S.S., Dewiyanti, I., & Rizwan, T. (2016). Identifikasi Jenis Ikan di Perairan Laguna Gampoeng Pulot Kecamatan Leupung Aceh Besar. Jurnal Ilmiah Mahasiswa Kelautan Perikanan Unsyiah, 1(1), 66-81.
Golenberg, E.M., Bickel, A., & Weihs, P. (1996). Effect of Highly Fragmented DNA on PCR. Nucleic Acids Research, 24(24), 5026-5033. https://doi.org/10.1093/nar/24.24.5026.
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). Mega X: Molecular Evolutionary Genetic Analysis Across Computing Platform. Molecular Biology Evolution, 35(6), 1547-1549. DOI: 10.1093/molbev/msy096.
Lee, W., Conroy, J., Howell, W.H., & Kocher, T.D., 1995. Structure and Evolution of Teleost Mitochondrial Control Regions. Journal of Molecular Evolution, 41, 54-66.
Loiseau, N., & Gaertner, J. (2015). Indices for Assessing Coral Reef Fish Biodiversity: The Need for A Change in Habits. Ecology and Evolution, 5(18), 4018-4027. doi: 10.1002/ece3.1619.
Lubis, E.K., Sinaga, T.Y., & Susiana. (2021). Inventarisasi Ikan Demersal dan Ikan Pelagis yang Didaratkan di PPI Kijang Kecamatan Bintan Timur Kabupaten Bintan. Jurnal Akuatiklestari, 4(2), 47-57. Doi: https://doi.org/10.31629/akuatiklestari.v4i2.2536.
Nei, M. (2013). Genetic Distance. Brenner’s Encyclopedia of Genetics (Second Edition). Academic Press. Pages 248-250. ISBN. 780080961569. https://doi.org/10.1016/B978-0-12-374984-0.00615-X.
Newell, P.D., Fricker, A.D., Roco, C.A., Chandrangsu, P., & Merkel, S.M. (2013). A Small-Group Activity Introducing the Use and Interpretation of BLAST. Journal of Microbiology & Biology Education. 14(2), 238-243. doi: 10.1128/jmbe.v14i2.637.
Nugroho, E.D., & Rahayu, D.A. (2015). Status Taksonomi Ikan Nomei dari Perairan Tarakan, Kalimantan Utara berdasarkan Gen 16S Rrna sebagai Upaya Konservasi Ikan Laut Lokal Indonesia.
Pertiwi, N.P.D., Suhendro, M.D., Yusmalinda, N.L.A., Putra, I.N.G., Putri, I.G.R.M., Artiningsih, E.Y., Malik, M.D.A., Cahyani, N.K.D., & Sembiring, A. (2020). Forensic Genetic Case Study: Species Identification and Traceability of Sea Turtle Caught in Illegal Trade in Bali, Indonesia. Biodiversitas, 21(9), 4276-4283. DOI: 10.13057/biodiv/d210945.
Pramono, T.B., Arfati, D., Widodo, M.S., & Yanuhar, U. (2017). Identifikasi Ikan Genus Mystus dengan Pendekatan Genetik. Jurnal Sumberdaya Akuatik Indopasifik, 1(2), 123-132.
Reis, R.E., Albert, J.S., Darios, F.D., Mincarone, M.M., Pertry, P., & Rocha, L.A. (2016). Fish Biodiversity and Conservation in South America. Journal of Fish Biology, 89, 12-47. doi:10.1111/jfb.13016.
Romdon, A., Sembiring, A., Pertiwi, N.P.D., Malik, M.D.A., Pay, L., Hehuwat, Y., & Satrioajie, W.N. (2020). Description and Identification of Tuna Larvae Based On Genetic and Morphological Analysis. IOP Conference Series: Earth and Environmental Science, 618. doi:10.1088/1755-1315/618/1/012035.
Russo, C.A.D.M., & Selvatti, A.P. (2018). Bootstrap and Rogue Identification Tests for Phylogenetic Analysis. Molecular Biology and Evolution, 35(9), 2327-2333. https://doi.org/10.1093/molbev/msy118.
Sembiring, A., Pertiwi, N.P.D., Mahardini, A., Wulandari, R., Kurniasih, E.M., Kuncoro, A.W., Cahyani, N.K.D., Anggoro, A.W., Ulfa, M., Maduppa, H., Carpenter, K.E., Barber, P.H., & Mahardika, G.N. (2015). Short Communication: DNA Barcoding Reveals Targeted Fisheries for Endangered Sharks in Indonesia. Fisheries Research, 164, 130-234. http://dx.doi.org/10.1016/j.fishres.2014.11.003.
Shafait, F., Mian, A., Shortis, M., Ghanes, B., Culverhouse, P.F., Edgington, D., Cline, D., Ravanbanksh, M., Saeger, J., & Harvey, E.S. (2016). Fish Identification from Videos Captured in Uncontrolled Underwater Environments. ICES Journal of Marine Science, 73(10), 2737-2747. doi:10.1093/icesjms/fsw106.
Walsh, P.S., Metzger, D.A., & Higuchi, R. (1991). Chelex-100 as A Medium for Simple Extraction of DNA for PCR Based Typing from Forensic Material. Biotechniques, 10, 506-513.
Yonvitner, Boer, M., & Kurni, R. (2020). Kajian Tingkat Efektifitas Perikanan untuk Pengembangan secara Berkelanjutan di Provinsi Banten. Jurnal Kebijakan Perikanan Indonesia, 12(2), 35-46. DOI: http://dx.doi.org/10.15578/jkpi.12.1.2020.35-46.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Jurnal Pendidikan Biologi undiksha
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Pendidikan Biologi Undiksha is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
