ANALISIS STRUKTUR GEN DAN PROTEIN LIPID DROPLET-ASSOCIATED PROTEIN (LDAP) YANG BERPERAN DALAM BIOGENESIS MINYAK PADA TANAMAN JARAK PAGAR (Jatropha curcas L.)

Fredy Saudale

Abstract


Tanaman Jarak Pagar atau Jatropha curcas adalah salah satu sumber biodiesel yang menawarkan keuntungan langsung dan berkelanjutan terhadap efek rumah kaca. Ekspresi protein lipid droplet-associated protein (LDAP) berkorelasi dengan tingginya akumulasi lemak, baik di biji maupun buah. Dalam studi yang menggunakan metode penjajaran sekuen jamak ini, LDAP Jatropha menunjukkan tingkat identitas yang tinggi sebesar 82.92% dengan LDAP tanaman karet; 67,50% dengan LDAP3 Arabidopsis; 61,21% dengan LDAP2 buah Alpukat, dan 55,65% dengan LDAP1 buah Alpukat. Namun LDAP Jatropha menunjukkan tingkat identitas yang rendah yaitu sebesar 46,01% dan 37,04% masing-masing dengan LDAP2 dan LDAP1 Arabidopsis. Struktur gen dari LDAP Jatropha terdiri atas 3 ekson dan 2 intron. Asam amino pada posisi 20-40 menunjukkan indeks hidropobisitas yang tinggi (=2) dan diprediksi berinteraksi dengan LDAP dalam memfasilitasi proses biogenesis minyak pada tanaman jarak pagar.


Keywords


Biodiesel; jatropha; lipid droplet; LDAP

Full Text:

PDF

References


Bateman, A., Coin, L., Durbin, R., Finn, R. D., Hollich, V., Griffiths-Jones, S., Khanna, A., Marshall, M., Moxon, S., & Sonnhammer, E. L. (2004). The Pfam protein families database. Nucleic Acids Research, 32(suppl_1), D138–D141.

Berchmans, H. J., & Hirata, S. (2008). Biodiesel production from crude Jatropha curcas L. seed oil with a high content of free fatty acids. Bioresource Technology, 99(6), 1716–1721.

Brinkman, F. S., Wan, I., Hancock, R. E., Rose, A. M., & Jones, S. J. (2001). PhyloBLAST: Facilitating phylogenetic analysis of BLAST results. Bioinformatics, 17(4), 385–387.

Chapman, K. D., Dyer, J. M., & Mullen, R. T. (2012). Biogenesis and functions of lipid droplets in plants thematic review series: Lipid droplet synthesis and metabolism: from yeast to man. Journal of Lipid Research, 53(2), 215–226.

Chenna, R., Sugawara, H., Koike, T., Lopez, R., Gibson, T. J., Higgins, D. G., & Thompson, J. D. (2003). Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Research, 31(13), 3497–3500.

Cruz, A. L., Carrossini, N., Teixeira, L. K., Ribeiro-Pinto, L. F., Bozza, P. T., & Viola, J. P. (2019). Cell cycle progression regulates biogenesis and cellular localization of lipid droplets. Molecular and Cellular Biology, 39(9), e00374–18.

Damodharan, L., & Pattabhi, V. (2004). Hydropathy analysis to correlate structure and function of proteins. Biochemical and Biophysical Research Communications, 323(3), 996–1002.

D’Onofrio, G., Jabbari, K., Musto, H., & Bernardi, G. (1999). The correlation of protein hydropathy with the base composition of coding sequences. Gene, 238(1), 3–14.

Durrett, T. P., Benning, C., & Ohlrogge, J. (2008). Plant triacylglycerols as feedstocks for the production of biofuels. The Plant Journal, 54(4), 593–607.

Eboibi, B. E., Eboibi, O., Okputu, J., & Okpohwo, K. A. (2018). Production and analysis of biodiesel from Jatropha curcas seed. Journal of Applied Sciences and Environmental Management, 22(1), 26–33.

Felber, J.-P., & Golay, A. (1995). Regulation of nutrient metabolism and energy expenditure. Metabolism, 44, 4–9.

Fuentes, A., García, C., Hennecke, A., & Masera, O. (2018). Life cycle assessment of Jatropha curcas biodiesel production: A case study in Mexico. Clean Technologies and Environmental Policy, 20(7), 1721–1733.

Gasteiger, E., Hoogland, C., Gattiker, A., Wilkins, M. R., Appel, R. D., & Bairoch, A. (2005). Protein identification and analysis tools on the ExPASy server. In The proteomics protocols handbook (pp. 571–607). Springer.

Ghosh, P., Westhoff, P., & Debnath, D. (2019). Biofuels, food security, and sustainability. In Biofuels, Bioenergy and Food Security (pp. 211–229). Elsevier.

Gidda, S. K., Park, S., Pyc, M., Yurchenko, O., Cai, Y., Wu, P., Andrews, D. W., Chapman, K. D., Dyer, J. M., & Mullen, R. T. (2016). Lipid droplet-associated proteins (LDAPs) are required for the dynamic regulation of neutral lipid compartmentation in plant cells. Plant Physiology, 170(4), 2052–2071.

Gidda, S. K., Watt, S. C., Collins-Silva, J., Kilaru, A., Arondel, V., Yurchenko, O., Horn, P. J., James, C. N., Shintani, D., & Ohlrogge, J. B. (2013). Lipid droplet-associated proteins (LDAPs) are involved in the compartmentalization of lipophilic compounds in plant cells. Plant Signaling & Behavior, 8(11), e27141.

Goodman, J. M. (2008). The gregarious lipid droplet. Journal of Biological Chemistry, 283(42), 28005–28009.

Greenway, H., & Gibbs, J. (2003). Mechanisms of anoxia tolerance in plants. II. Energy requirements for maintenance and energy distribution to essential processes. Functional Plant Biology, 30(10), 999–1036.

Gübitz, G. M., Mittelbach, M., & Trabi, M. (1999). Exploitation of the tropical oil seed plant Jatropha curcas L. Bioresource Technology, 67(1), 73–82.

Hood, E. E. (2016). Plant-based biofuels. F1000Research, 5.

Horn, P. J., James, C. N., Gidda, S. K., Kilaru, A., Dyer, J. M., Mullen, R. T., Ohlrogge, J. B., & Chapman, K. D. (2013). Identification of a new class of lipid droplet-associated proteins in plants. Plant Physiology, 162(4), 1926–1936.

Jones, D. T., Taylor, W. R., & Thornton, J. M. (1992). The rapid generation of mutation data matrices from protein sequences. Bioinformatics, 8(3), 275–282.

Lu, H., Liu, Y., Zhou, H., Yang, Y., Chen, M., & Liang, B. (2009). Production of biodiesel from Jatropha curcas L. oil. Computers & Chemical Engineering, 33(5), 1091–1096.

Martin, S., & Parton, R. G. (2006). Opinion: Lipid droplets: a unified view of a dynamic organelle. Nature Reviews Molecular Cell Biology, 7(5), 373-378.

McGinnis, S., & Madden, T. L. (2004). BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Research, 32(2), W20–W25.

Meyer, E., Logan, T. L., & Juenger, T. E. (2012). Transcriptome analysis and gene expression atlas for Panicum hallii var. Filipes, a diploid model for biofuel research. The Plant Journal, 70(5), 879–890.

Mohanty, S. K., & Swain, M. R. (2019). Bioethanol Production From Corn and Wheat: Food, Fuel, and Future. In Bioethanol Production from Food Crops (pp. 45–59). Elsevier.

Olzmann, J. A., & Carvalho, P. (2019). Dynamics and functions of lipid droplets. Nature Reviews Molecular Cell Biology, 20(3), 137–155.

Openshaw, K. (2000). A review of Jatropha curcas: An oil plant of unfulfilled promise. Biomass and Bioenergy, 19(1), 1–15.

Powlson, D. S., Riche, A. B., & Shield, I. (2005). Biofuels and other approaches for decreasing fossil fuel emissions from agriculture. Annals of Applied Biology, 146(2), 193–201.

Pramanik, K. (2003). Properties and use of Jatropha curcas oil and diesel fuel blends in compression ignition engine. Renewable Energy, 28(2), 239–248.

Saudale, F., & Boelan, E. (2018). Aktivitas Antibakteri Ekstrak Polar dan Non Polar Biji Kelor (Moringa oleifera) Asal Pulau TImor NTT. Jurnal Sains Dan Teknologi, 7(1), 67–76.

Sievers, F., & Higgins, D. G. (2018). Clustal Omega for making accurate alignments of many protein sequences. Protein Science, 27(1), 135–145.

Sievers, F., Wilm, A., Dineen, D., Gibson, T. J., Karplus, K., Li, W., Lopez, R., McWilliam, H., Remmert, M., Soding, J., Thompson, J. D., & Higgins, D. G. (2011). Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega. Molecular Systems Biology, 7, 539.

Silitonga, A. S., Atabani, A. E., Mahlia, T. M. I., Masjuki, H. H., Badruddin, I. A., & Mekhilef, S. (2011). A review on prospect of Jatropha curcas for biodiesel in Indonesia. Renewable and Sustainable Energy Reviews, 15(8), 3733–3756.

Tiwari, A. K., Kumar, A., & Raheman, H. (2007). Biodiesel production from jatropha oil (Jatropha curcas) with high free fatty acids: An optimized process. Biomass and Bioenergy, 31(8), 569–575.

Ye, J., McGinnis, S., & Madden, T. L. (2006). BLAST: Improvements for better sequence analysis. Nucleic Acids Research, 34(suppl_2), W6–W9.




DOI: http://dx.doi.org/10.23887/jst-undiksha.v9i2.21327

Article Metrics

Abstract view : 266 times
PDF file view : 109 times

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 JST (Jurnal Sains dan Teknologi)

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


Jurnal Sains dan Teknologi (JST) is published by:

LEMBAGA PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT (LPPM)

UNIVERSITAS PENDIDIKAN GANESHA

Jl. Udayana, Kampus Tengah, Singaraja-Bali
Kode Pos 81116
Telp. 0362-22928
Homepage: https://lppm.undiksha.ac.id
Email: wayanwidiana85@undiksha.ac.id


 

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License