Antimicrobial Activity of Basil (Ocimum basilicum L.) Against Gram-Negative Bacteria Involved in Pneumonia Infection

Penulis

  • Indah Sulistiyawati Universitas Nahdlatul Ulama, Purwokerto, Indonesia
  • Muhammad Falah Universitas Nahdlatul Ulama, Purwokerto, Indonesia
  • Gita Anggraeni Universitas Nahdlatul Ulama, Purwokerto, Indonesia
  • Anwar Rovik Universitas Gadjah Mada, Yogyakarta, Indonesia

DOI:

https://doi.org/10.23887/jstundiksha.v12i2.53577

Kata Kunci:

Antimicrobial resistance, infectious diseases, Ocimum basilicum, phytochemical, pneumonia

Abstrak

Infectious diseases are still a global problem, for example, acute respiratory infections (SARI). Resistance in pneumonia involving Gram-negative bacterial infection reduces the effectiveness of antibiotic use and increases the length of hospital stay. This study examines the potential of antimicrobial compounds from basil plants against isolates of Gram-negative bacteria involved in pneumonia infection. This research is an experimental study through several stages: basil extraction, initial screening of bioactive compounds, isolation and identification of bacteria from sputum, and testing for antimicrobial activity of basil extracts. Sputum samples were collected from 43 pneumonia patients (male and female) involving Klebsiella pneumoniae, Acinetobacter baumannii, Enterobacter aerogenes, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Escherichia coli. The inhibitory activity against pathogenic bacteria was directly proportional to the basil extract concentration. Initial screening for phytochemical compounds showed that the extracts of basil leaves and stems contained active compounds of tannins and flavonoids. The 25% concentration of basil extract was the minimum inhibitory concentration (MIC) against the test bacteria. The 100% basil extract concentration produced the largest inhibition zone i.e., 10.93 mm. The overall findings of this study provide baseline information for the possible use of the Ocimum basilicum extract in the treatment of pneumonia involving Gram-negative bacteria.

Referensi

Akram, A. R., Chankeshwara, S. V., Scholefield, E., Aslam, T., McDonald, N., Megia-Fernandez, A., Marshall, A., Mills, B., Avlonitis, N., Craven, T. H., Smyth, A. M., Collie, D. S., Gray, C., Hirani, N., Hill, A. T., Govan, J. R., Walsh, T., Haslett, C., Bradley, M., & Dhaliwal, K. (2018). In situ identification of Gram-negative bacteria in human lungs using a topical fluorescent peptide targeting lipid A. Science Translational Medicine, 10(464). https://doi.org/10.1126/scitranslmed.aal0033.

Aman, A. T., Wibawa, T., Kosasih, H., Asdie, R. H., Safitri, I., Intansari, U. S., Mawarti, Y., Sudarmono, P., Arif, M., Puspitasari, D., Alisjahbana, B., Parwati, K. T. M., Gasem, M. H., Lokida, D., Lukman, N., Hartono, T. S., Mardian, Y., Liang, C. J., Siddiqui, S., … Lau, C. Y. (2021). Etiologies of severe acute respiratory infection (SARI) and misdiagnosis of influenza in Indonesia, 2013-2016. Influenza and Other Respiratory Viruses, 15(1), 34–44. https://doi.org/10.1111/irv.12781.

Aminian, A. R., Mohebbati, R., & Boskabady, M. H. (2022). The Effect of Ocimum basilicum L. and Its Main Ingredients on Respiratory Disorders: An Experimental, Preclinical, and Clinical Review. Frontiers in Pharmacology, 12(January), 1–14. https://doi.org/10.3389/fphar.2021.805391.

Ariani, N., Febrianti, D. R., & Niah, R. (2020). Uji Aktivitas Ekstrak Etanolik Daun Kemangi (Ocimum sanctum L.) terhadap Staphylococcus aureus secara In Vitr. Jurnal Pharmascience, 7(1), 107. https://doi.org/10.20527/jps.v7i1.8080.

Assefa, M. (2022). Multi-drug resistant gram-negative bacterial pneumonia: etiology, risk factors, and drug resistance patterns. Pneumonia, 14(1). https://doi.org/10.1186/s41479-022-00096-z.

Christaki, E., Marcou, M., & Tofarides, A. (2019). Antimicrobial Resistance in Bacteria : Mechanisms , Evolution , and Persistence. Journal of Molecular Evolution, 0123456789. https://doi.org/10.1007/s00239-019-09914-3.

da Costa, R. L., Lamas, C. da C., Simvoulidis, L. F. N., Espanha, C. A., Moreira, L. P. M., Bonancim, R. A. B., Weber, J. V. L. A., Ramos, M. R. F., Silva, E. C. de F., & de Oliveira, L. P. (2022). Secondary infections in a cohort of patients with COVID-19 admitted to an intensive care unit: impact of gram-negative bacterial resistance. Revista Do Instituto de Medicina Tropical de Sao Paulo, 64, 1–10. https://doi.org/10.1590/S1678-9946202264006.

Dunstan, R. A., Bamert, R. S., Belousoff, M. J., Short, F. L., Barlow, C. K., Pickard, D. J., Wilksch, J. J., Schittenhelm, R. B., Strugnell, R. A., Dougan, G., & Lithgow, T. (2021). Mechanistic Insights into the Capsule-Targeting Depolymerase from a Klebsiella pneumoniae Bacteriophage. Microbiology Spectrum, 9(1), 1–15. https://doi.org/10.1128/spectrum.01023-21.

Eftekhar, N., Moghimi, A., Mohammadian Roshan, N., Saadat, S., & Boskabady, M. H. (2019). Immunomodulatory and anti-inflammatory effects of hydro-ethanolic extract of Ocimum basilicum leaves and its effect on lung pathological changes in an ovalbumin-induced rat model of asthma. BMC Complementary and Alternative Medicine, 19(1), 1–11. https://doi.org/10.1186/s12906-019-2765-4.

Epand, R. M., Walker, C., Epand, R. F., & Magarvey, N. A. (2016). Molecular mechanisms of membrane targeting antibiotics. Biochimica et Biophysica Acta - Biomembranes, 1858(5), 980–987. https://doi.org/10.1016/j.bbamem.2015.10.018.

Favour, O., Rm, M., Taiwo, S., Alli, O., Do, O., Eo, A., & Anukam, K. (2014). Klebsiella has taken lead among uropathogens in University of Benin Teaching Hospital, Benin City, Nigeria-An observation. New York Science Journal, 3(11), 61–64. https://www.researchgate.net/profile/Hamza-Sule/publication/304339309.

Gaio, I., Saggiorato, A. G., Treichel, H., Cichoski, A. J., Astolfi, V., Cardoso, R. I., Toniazzo, G., Valduga, E., Paroul, N., & Cansian, R. L. (2015). Antibacterial activity of basil essential oil (Ocimum basilicum L.) in Italian-type sausage. Journal Fur Verbraucherschutz Und Lebensmittelsicherheit, 10(4), 323–329. https://doi.org/10.1007/s00003-015-0936-x

Harapan, I. K., Tahulending, A., & Tumbol, M. V. L. (2018). Karakteristik Resistensi Klebsiella pneumoniae Yang Resisten Karbapenem Pada Beberapa Rumah Sakit Di Indonesia Dan Pemeriksaan Laboratorium. Prosiding Seminar Nasional Tahun 2018 Menuju Masyarakat Sehat, Berkarakter Dan Berprestasi, 1(3), 636–650. https://mail.ejurnal.poltekkes-manado.ac.id/index.php/prosiding2018/article/view/480.

Hasani, A., Soltani, E., Rezaee, M. A., Pirzadeh, T., Oskouee, M. A., Hasani, A., Gholizadeh, P., Oskouie, A. N., & Binesh, E. (2020). Serotyping of klebsiella pneumoniae and its relation with capsule-associated virulence genes, antimicrobial resistance pattern, and clinical infections: A descriptive study in medical practice. Infection and Drug Resistance, 13, 1971–1980. https://doi.org/10.2147/IDR.S243984.

Ikram, A., Saleem, S., Imran, M., & Ghazal, A. (2021). Antimicrobial Activity by Solvents Extracted from Ocimum Basilicum Herb Against Multidrug Resistant Gram-Negative Rods. 200–206.

Khair-ul-Bariyah, S., Ahmed, D., & Ikram, M. (2012). Ocimum Basilicum: A Review on Phytochemical and Pharmacological Studies. Pakistan Journal of Chemistry, 2(2), 78–85. https://doi.org/10.15228/2012.v02.i02.p05.

Lemos, I. C. S., De Araújo Delmondes, G., Ferreira Dos Santos, A. D., Santos, E. S., De Oliveira, D. R., De Figueiredo, P. R. L., De Araújo Alves, D., Barbosa, R., De Menezes, I. R. A., Coutinho, H. D., Kerntop, M. R., & Fernandes, G. P. (2016). Ethnobiological survey of plants and animals used for the treatment of acute respiratory infections in children of a traditional community in the municipality of barbalha, cearÁ, Brazil. African Journal of Traditional, Complementary and Alternative Medicines, 13(4), 166–175. https://doi.org/10.21010/ajtcam.v13i4.22.

Lim, Y. K., Kweon, O. J., Kim, H. R., Kim, T. H., & Lee, M. K. (2019). Impact of bacterial and viral coinfection in community-acquired pneumonia in adults. Diagnostic Microbiology and Infectious Disease, 94(1), 50–54. https://doi.org/10.1016/j.diagmicrobio.2018.11.014.

Loutet, S. A., & Valvano, M. A. (2011). Extreme Antimicrobial Peptide and Polymyxin B Resistance in the Genus Burkholderia. Frontiers in Microbiology, 2(July), 1–8. https://doi.org/10.3389/fmicb.2011.00159.

Martin-Loeches, I., Rodriguez, A. H., & Torres, A. (2018). New guidelines for hospital-acquired pneumonia/ ventilator-associated pneumonia: USA vs. Europe. Current Opinion in Critical Care, 24(5), 347–352. https://doi.org/10.1097/MCC.0000000000000535.

Masturoh, I., & Anggita, N. T. (2018). Metodologi Penelitian Kesehatan. In Kementerian Kesehatan Republik Indonesia Pusat Pendidikan Sumber Daya Manusia Kesehatan Badan Pengembangan dan Pemberdayaan Sumber Daya Manusia Kesehatan.

Melloni, B., Laaban, J., & Chambellan, A. (2018). Home-Based Care Evolution in Chronic Respiratory Failure between 2001 and 2015 ( Antadir Federation Observatory ). 446–454. https://doi.org/10.1159/000490549.

Mittal, R., Kumar, R., & Chahal, H. (2018). Antimicrobial activity of Ocimum sanctum leaves extracts and oil. Journal of Drug Delivery and Therapeutics, 8(6), 201–204. https://doi.org/10.22270/jddt.v8i6.2166.

Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt Dengan Metode Difusi Sumuran Dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41. https://doi.org/10.24198/jthp.v1i2.27537.

Ong, C. W. M., Lye, D. C. B., Khoo, K. L., Chua, G. S. W., Yeoh, S. F., Leo, Y. S., Tambyah, P. A., & Chua, A. C. (2009). Severe community-acquired Acinetobacter baumannii pneumonia: An emerging highly lethal infectious disease in the Asia-Pacific. Respirology, 14(8), 1200–1205. https://doi.org/10.1111/j.1440-1843.2009.01630.x.

Opoku-Temeng, C., Kobayashi, S. D., & DeLeo, F. R. (2019). Klebsiella pneumoniae capsule polysaccharide as a target for therapeutics and vaccines. Computational and Structural Biotechnology Journal, 17, 1360–1366. https://doi.org/10.1016/j.csbj.2019.09.011.

Pandey, A. K., Singh, P., & Tripathi, N. N. (2014). Chemistry and bioactivities of essential oils of some Ocimum species: An overview. Asian Pacific Journal of Tropical Biomedicine, 4(9), 682–694. https://doi.org/10.12980/APJTB.4.2014C77.

Purba, A. K., Ascobat, P., Muchtar, A., Wulandari, L., Rosyid, A. N., Purwono, P. B., Van Der Werf, T. S., Friedrich, A. W., & Postma, M. J. (2019). Multidrug-resistant infections among hospitalized adults with community-acquired pneumonia in an Indonesian tertiary referral hospital. Infection and Drug Resistance, 12, 3663–3675. https://doi.org/10.2147/IDR.S217842.

Pushpalatha., M., Ponvel., M., & P.C. Sathya, N. (2021). Phytochemical Screening And Antioxidant, Antibacterial Activities Of Ocimum basilicum L. Journal of Cardiovascular Disease Research, 12(01), 269–281. https://www.jcdronline.org/admin/Uploads/Files/6242e2c17ce377.91613361.pdf.

Rezzoug, M., Bakchiche, B., Gherib, A., Roberta, A., Guido, F., Kilinçarslan, Ö., Mammadov, R., & Bardaweel, S. K. (2019). Chemical composition and bioactivity of essential oils and Ethanolic extracts of Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut. from the Algerian Saharan Atlas. BMC Complementary and Alternative Medicine, 19(1), 1–10. https://doi.org/10.1186/s12906-019-2556-y.

Rustam, Z., Yuda, R. P., Alatas, H., & Aroef, C. (2020). Pulmonary rontgen classification to detect pneumonia disease using convolutional neural networks. Telkomnika (Telecommunication Computing Electronics and Control), 18(3), 1522–1528. https://doi.org/10.12928/TELKOMNIKA.v18i3.14839.

Salleh, N. N., Yahya, H., Ariffin, N., & Yahya, H. N. (2021). Antibacterial Properties of Ocimum Spp . ( Ocimum Basilicum L . and Ocimum Basilicum Var . Purpurascens ) Against Selected Bacteria Nurul Nadia Binti Salleh. 4472(10), 194–200. https://doi.org/10.36349/easjals.2021.v04i10.001.

Santoso, P., Sung, M., Hartantri, Y., Andriyoko, B., Sugianli, A. K., Alisjahbana, B., Tjiam, J. S. L., Debora, J., Kusumawati, D., & Soeroto, A. Y. (2022). MDR Pathogens Organisms as Risk Factor of Mortality in Secondary Pulmonary Bacterial Infections Among COVID-19 Patients: Observational Studies in Two Referral Hospitals in West Java, Indonesia. International Journal of General Medicine, 15(May), 4741–4751. https://doi.org/10.2147/IJGM.S359959.

Song, L., Hu, X., Ren, X., Liu, J., & Liu, X. (2022). Antibacterial Modes of Herbal Flavonoids Combat Resistant Bacteria. Frontiers in Pharmacology, 13(June), 1–11. https://doi.org/10.3389/fphar.2022.873374.

Tsakiridou, E., Makris, D., Daniil, Z., Manoulakas, E., Chatzipantazi, V., Vlachos, O., Xidopoulos, G., Charalampidou, O., & Zakynthinos, E. (2014). Acinetobacter baumannii infection in prior ICU bed occupants is an independent risk factor for subsequent cases of ventilator-associated pneumonia. BioMed Research International, 2014. https://doi.org/10.1155/2014/193516.

Versporten, A., Zarb, P., Caniaux, I., Gros, M. F., Drapier, N., Miller, M., Jarlier, V., Nathwani, D., Goossens, H., Koraqi, A., Hoxha, I., Tafaj, S., Lacej, D., Hojman, M., Quiros, R. E., Ghazaryan, L., Cairns, K. A., Cheng, A., Horne, K. C., … May, S. (2018). Antimicrobial consumption and resistance in adult hospital inpatients in 53 countries: results of an internet-based global point prevalence survey. The Lancet Global Health, 6(6), e619–e629. https://doi.org/10.1016/S2214-109X(18)30186-4.

Windels, E. M., Michiels, J. E., van den Bergh, B., Fauvart, M., & Michiels, J. (2019). Antibiotics: Combatting tolerance to stop resistance. MBio, 10(5). https://doi.org/10.1128/mBio.02095-19.

Yadav, M., Chatterji, S., Gupta, K, S., & Watal, G. (2014). Preliminary phytochemical screening of six medicinal plants used as traditional medicine. International Journal of Pharma and Bio Sciences, 6(5), 539–542. https://www.academia.edu/download/51053081/mp.pdf.

Yamasaki, S., Shigemura, K., Osawa, K., Kitagawa, K., Ishii, A., Kuntaman, K., Shirakawa, T., Miyara, T., & Fujisawa, M. (2021). Genetic analysis of ESBL-producing Klebsiella pneumoniae isolated from UTI patients in Indonesia. Journal of Infection and Chemotherapy, 27(1), 55–61. https://doi.org/10.1016/j.jiac.2020.08.007.

Unduhan

Diterbitkan

2023-10-22

Terbitan

Vol 12 No 2 (2023): July

Bagian

Articles

Lisensi

Hak Cipta (c) 2023 Indah Sulistiyawati, Muhammad Falah, Gita Anggraeni; Anwar Rovik

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