PRODUKSI PIGMEN WARNA MERAH DARI JAMUR PENICILLIUM PURPUROGENUM YANG DIISOLASI DARI TANAH TERCEMAR LIMBAH SUSU KAMBING DENGAN METODE SUBMERGED FERMENTATION
DOI:
https://doi.org/10.23887/wms.v11i1.11844Keywords:
ampas parutan kelapa, pigmen merah, Penicillium purpurogenumAbstract
Penelitian ini bertujuan untuk menentukan kondisi optimum produksi pigmen warna merah dari jamur Penicillium purpurogenum yang diisolasi dari tanah tercemar limbah susu kambing. Produksi pigmen menggunakan metode submerged fermentation pada media PD Broth dengan variasi solid support (ampas kelapa dan rumput laut), suhu (30oC, 35oC, 40oC, dan 45oC), pH (4-10) sumber karbon (glukosa, sukrosa, dan pati) dan sumber nitrogen (ekstrak ragi dan pepton). Pigmen merah yang dihasilkan diekstrak dengan menggunakan akuades dan diukur nilai absorbansinya menggunakan spektrofotometer UV-Vis model 1800 pada panjang gelombang 490 nm. Jumlah pigmen yang dihasilkan, direpresentasikan oleh nilai absorbansi pigmen pada setiap kondisi yang terukur. Hasil penelitian menunjukkan bahwa produksi pigmen warna merah dari jamur Penicillium purpurogenum berlangsung optimum menggunakan media PDB yang disuplementasi ampas parutan kelapa, sukrosa 2%, ektrak ragi 2% yang dikondisikan pada suhu 30oC,dan pH 5 dengan lama waktu inkubasi 12 hari.References
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