Isolasi Bakteri Endofit dari Tanaman Laban (Vitex Pubescens Vahl) sebagai Antibakteri
DOI:
https://doi.org/10.23887/jstundiksha.v11i1.42781Abstrak
Pemanfaatan bakteri endofit sebagai penghasil senyawa metabolit sekunder sangat menguntungkan. Kandungan metabolit sekunder yang terdapat di dalam jaringan tumbuhan serta dalam bakteri merupakan potensi yang memberikan banyak manfaat dalam bidang pertanian. Penelitian ini bertujuan untuk mengisolasi bakteri endofit dari daun dan kulit batang tanaman Laban (Vitex pubescens Vahl) dan uji antibakteri. Bakteri diisolasi menggunakan media HVA. Karakteristik bakteri endofit diamati secara makroskopis, mikroskopis, dan melakukan uji katalase serta untuk uji antibakteri menggunakan bakteri patogen Erwinia carotrova. Jumlah isolat endofit yang berhasil diisolasi berjumlah delapan isolat (empat isolat dari daun laban dan empat isolat dari kulit batang laban). Karakterisasi dilakukan pada delapan isolat endofit yang dihasilkan bahwa isolat memiliki ciri-ciri yang berbeda baik secara makroskopis, mikroskopis, dan semua isolat endofit dari tanaman laban menghasilkan enzim katalase (uji +). Hasil aktivitas antibakteri menunjukkan tujuh isolat bakteri endofit memiliki aktivitas antibakteri terhadap erwinia carotrovora. Tanaman laban kaya akan keragaman bakteri endofit. Isolate bakteri endofit terisolasi 8 spesies yang berbeda (DA45, DA46, DB15, DB16, KBC1, KBC2, KBC3 dan KBC4). Bakteri endofit yang berasosiasi dengan tanaman berperan penting dalam kesehatan tanaman.
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